Discipline of Virology

Research

Project Title: Occult Hepatitis B Virus infection in haemodialysis units-Durban, South Africa

Researcher: Dr NB Msomi

Co-Researchers: Ndlovu K (UKZN/DoH), Mlisana K (UKZN/NHLS);

Collaborators: Ijaz S (Public Health England), Giandhari J (KRISP-UKZN);

Project Title: Hepatitis B virus incidence in patients seeking HIV care- CAPRISA Antiretroviral Treatment Program cohort

Principal Investigator: Dr NB Msomi

Co-Researchers: Naidoo K (CAPRISA), Yende-Zuma N (CAPRISA); Mlisana K (UKZN/NHLS)

Project Title: Aptamer-functionalized carbosilane dendrimers to reactivate HIV-1 latency

Principal Investigator: Dr R Parboosing

Collaborator: Aptus Biotech (Spain)

Project Title: Highly Active siRNAs for the treatment of HIV infection

Principal Investigator: Dr R Parboosing

Project Title: Eradication of HIV-1 from latent cellular reservoirs: An in vitro proof of concept study

Principal Investigator: Dr R Parboosing

Project Title: Hepatitis B Virus variants in HBV mono-infected and HIV/HBV co-infected patients in a high dual infection setting

Principal Investigator: Dr N Msomi

Co-Researchers: Moodley P, Parboosing R, Mlisana K (UKZN/NHLS)

Collaborators: Ijaz S (Public Health England), de Oliviera T, Giandhari J (KRISP)

Project Title: The role of evolving molecular technologies and compartmentalization in the clinical diagnosis of cytomegalovirus end organ disease in immunocompromised patients

Principal Investigators: Govender K (UKZN)

Co-Researchers: Parboosing R (UKZN)

Project Title: Cytomegalovirus pneumonitis in infants in KwaZulu Natal, South Africa and compartmentalization in the lung

Principal Investigators: Govender K (UKZN)

Co-Researchers: Parboosing R (UKZN) Moodley P (UKZN) Gordon M (UKZN)

Collaborators: Suarez N (U Glasgow)

Project Title: Impact of Shifts to Birth Testing on Early Infant Diagnosis Program Outcomes in KwaZulu-Natal, South Africa

Principal Investigators: Smith, S (Columbia University)

Co-Researchers: Govender, K (UKZN) Moodley, P (UKZN) La Russa, P (Columbia University); Kuhn, L. (Columbia University) Archary M (UKZN)

Project Title: Targeted delivery of HIV-1 integrase inhibitors using nanoparticles

Principal Investigator: L Singh – PhD project

Collaborator: Prof. T Govender (Catalysis and Peptide Research Unit, UKZN)

Project Title: Impact of primary human immunodeficiency virus drug resistant mutations on antiretroviral therapy

Principal Investigator: B Chimukangrar – PhD project

Collaborators: Dr P Moodley and Dr R Samuel (Virology), Dr K Naidoo (CAPRISA), Prof. T de Oliveria (Africa Centre)

Project Title: Anaemia and pure cell aplasia secondary to Parvovirus in HIV-infected and un-infected patients

Researcher: Dr K-L A Francois – Virology MMed

Project Title: Prevalence and seasonality of common viral respiratory pathogens, including Cytomegalovirus in children, between 0–5 years of age in KwaZulu-Natal, an HIV endemic province in South Africa

Researcher: Dr T Famoroti – Virology MMed

Project Title: A Simple Laboratory Based Method Of Amplifying HIV Target Sequences Without Nucleic Acid Extraction In Patient Residual Blood Plasma Specimens

Supervisor: Dr Kerusha Govender

Project Title: Targeted delivery of siRNA into T-Lymphocytes using PEI-AuNP-Antibody complexes

Supervisor: Dr Raveen Parboosing

Project Title: Evaluation and comparison of hepatitis B Virus PCR with DNA extraction against Conventional PCR with no DNA extraction

Supervisor: Dr Nokukhanyi Msomi

Project Title: Target delivery of RNA Therapeutics by novel AntiCD32A-aptamer conjugates

Researcher: William Serumula PhD project

Supervisor: Dr Raveen Parboosing

Project Title: Effective delivery of siRNAs using carbosilane dendrimers in an acutely infected cell line

Researcher: Cassandra Soobramoney PhD Project

Supervisor: Dr Raveen Parboosing

Project Title: Good practice for Work Integrated Learning in Public Sector Medical Laboratories: Educational competencies of Training Supervisors

Researcher: Melini Baruth

Supervisor: Dr P Moodley

Project Title: Specimen rejection trends as a pre-analytical quality indicator at an Academic Virology Laboratory in KwaZulu – Natal

Researcher: Neshni Ramjugath

Supervisor: Dr P Moodley

Under Publications:  remove the 2011, 2012, 2013, 2014 and remove current projects.

Add

2017:

Singh L. et al. Development and evaluation of peptide-functionalized gold nanoparticles for HIV integrase inhibition. Int J Peptide Res Ther. 2018. DOI 10.1007/s10989-018-9673-1

Singh L. et al. The role of nanotechnology in the treatment of viral infections. Ther Adv Infect Dis. 2017; 4(4):105-131

Gounder L , Moodley P , Drain P.K, Hickey A.J and Moosa M.Y.S. Hepatic tuberculosis in human immunodeficiency virus co-infected adults: a case series of South African adults. BMC Infectious Diseases. 2017; 17(115). DOI 10.1186/s12879-017-2222-2

Parboosing R, Chonco L, de la Mata F. J, Govender T, Maguire G. E, Kruger H. G. Potential inhibition of HIV-1 encapsidation by oligoribonucleotide-dendrimer nanoparticle complexes. International Journal of Nanomedicine. 2017;12: 317-325

Allana S, Shashkina E, Mathema B, Bablishvili N, Tukvadze N, Shah NS, Kempker RR, Blumberg HM, Moodley P, Mlisana M, Brust JCM and Gandhi NR. pncA Gene Mutations Associated with Pyrazinamide Resistance in Drug-Resistant Tuberculosis, South Africa and Georgia. Emerging Infectious Diseases. 2017; 23(3).

Shah NS,Auld SC, Brust JCM, Mathema B, Ismail N, Moodley P, Mlisana M, Allana S, Campbell A, Mthiyane T, Morris N, Mpangase P, van der Meulen H, Omar SV, Brown TS, Narechania A, Shaskina E, Kapwata T, Kreiswirth B, Gandhi NR. Transmission of Extensively Drug-Resistant Tuberculosis in South Africa. N Engl J Med. 2017; 376:243-253

Singh L, Kruge HG, Maguire GEM, Govender T and Parboosing R. The role of nanotechnology in the treatment of viral infections. Ther Adv Infectious Dis. 2017:4 105-131

Siedner MJ, Bwana MB, Moosa MS, Paul M, Pillay S, McCluskey S, Aturinda I, Ard K, Muyindike W, Moodley P, Brijkumar J, Rautenberg T, George G, Johnson B, Gandhi RT, Sunpath H, Marconi VC. The REVAMP trial to evaluate HIV resistance testing in sub-Saharan Africa: a case study in clinical trial design in resource limited settings to optimize effectiveness and cost effectiveness estimates. HIV Clin Trials. 2017: 1-7. doi: 10.1080/15284336.2017.1349028.

Magee MJ, Yan V. Sun YV, Brust JCM, Shah NS, Ning Y, Allana S, Campbell A, Hui Q, Mlisana K, Moodley P, Gandhi NR. Polymorphisms in the vitamin D receptor gene are associated with reduced rate of sputum culture conversion in multidrug resistant tuberculosis patients in South Africa. PLOS ONE. https://doi.org/10.1371/journal.pone.0180916

Gillian H, Dokubo E, Takuva S, de Oliveira T, Ledwaba J, Dube N, Moodley P , Sabatier J, Deyde V, Morris L, Elliot R. Rates of Virological Suppression and Drug Resistance in Adult HIV-1 positive patients attending primary health care facilities in KwaZulu-Natal, South Africa. Journal of Antimicrobial Chemotherapy. https://doi.org/10.1093/jac/dkx252

Kapwata T, Morris N, Campbell A, Mthiyane T, Mpangase P, Nelson KN, Allana S, Brust JCM, Moodley P,Mlisana K, Gandhi NR, Shah NS. Spatial distribution of extensively drug-resistant tuberculosis (XDR TB) patients in KwaZulu-Natal, South Africa. PLOS ONE

Khan A. Need for advocacy for maternal immunization. S Afr Med J 2017;107(7):547.

Parboosing R, Govender T, Maguire GEM, Kruger HG. Synthesis, characterisation and biocompatibility of a multifunctional gold nanoparticle system for the delivery of single stranded RNA to lymphocytes. S. Afr. J. Chem. 2018; 71: 1-14

Jeena P, Govender K, Parboosing R, Adhikari M. Significance of cytomegalovirus in children with HIV associated pneumonia admitted to PICU International Journal of Tuberculosis and Lung Disease. 2017;21(12):1230-6

2016:

Mdlalose N, Parboosing R, Moodley, P The Prevalence of Hepatitis B virus infection in HIV-positive and HIV-negative infants: KwaZulu-Natal, South Africa African Journal of Laboratory Medicine 31 March 2016, Vol 5, No 1 (2016), 5 pages

Govender K, Parboosing R, Siyaca N, Moodley P Description of dried blood spot specimen quality and validation of a new method of specimen processing for HIV PCR African Journal of Laboratory Medicine 25 Feb 2016

Naidoo A, Parboosing R, Moodley P Optimized real time HCV PCR shows no evidence of Hepatitis C Virus in Dried Blood Spots from HIV-infected infants in KwaZulu-Natal, South Africa African Journal of Laboratory Medicine 18 March 2016

Rapid Urine LAM Testing Improves Diagnosis of Expectorated Smear-Negative Pulmonary Tuberculosis in an HIV-endemic Region, Drain, Paul K., Lilishia Gounder, Faieza Sahid, and Mahomed-Yunus S. Moosa. 2016. “Rapid Urine LAM Testing Improves Diagnosis of Expectorated Smear-Negative Pulmonary Tuberculosis in an HIV-endemic Region.” Scientific Reports 6 (1): 19992. doi:10.1038/srep19992. Nature scientific report

Brust JCM, Shah NS, Allana S, Mthiyane T, Mlisana K, Moodley P, Master I, Ning Y, Gandhi NR. Hearing loss and laboratory adverse events among patients with MDR TB and HIV co-infection receiving concurrent antiretroviral therapy. Poster. CROI 2016, Boston,USA.

Auld S, Brust JCM, Mathema B, Mthiyane T, Ismail N, Moodley P, McDaniel D, Allana S, Gandhi NR, Shah NS. HIV-associated XDR TB is Transmitted in Households and Hospitals in South Africa. Oral Presentation. CROI 2016, Boston, USA.

Govender T, Azumah R, Dutta J, Somboro A, Ramtahal M, Chonco L, Parboosing R, Bester L, Kruger G, Naicker T, Essack S (2016) In vitro evaluation of metal chelators as potential metallo--lactamase inhibitors Journal of Applied Microbiology 120(4):860-7.

Parboosing R, Mzobe G, Chonco L, Moodley I (2016) Cell-based Assays for Assessing Toxicity: A Basic Guide Medicinal Chemistry

Samuel R, Julian MN, Paredes R, Parboosing R, Moodley P, Singh L, Naidoo A, Gordon M. (2016) HIV-1 Drug resistance by Ultra-deep sequencing following short course Zidovudine, single dose Nevirapine and single dose Tenofovir with Emtricitabine for pMTCT Journal of Acquired Immune Deficiency Syndromes: JAIDS 2016 Dec 1;73(4):384-389.

Chetty NK, Ijumba NM, Chetty L, Govender T, Parboosing R, Davidson IE (2016) Analysis of Current Pulses in HeLa-Cell Permeabilization Due to High Voltage

DC Corona Discharge IEEE Transactions on NanoBioscience, IEEE TRANSACTIONS ON NANOBIOSCIENCE, VOL. 15, NO. 6, SEPTEMBER 2016

Singh L, Parboosing R, Kruger HG, Maguire GEM, Govender T (2016) Intracellular tracking of fluorescent gold nanoparticles with targeted nuclear delivery in MT4 lymphocytes Advances in Natural Sciences: Nanoscience and Nanotechnology Adv. Nat. Sci.: Nanosci. Nanotechnol. 7 (2016) 045013 (7pp)

Govender K, Jeena, P, Parboosing R (2016) The clinical utility of bronchoalveolar lavage cytomegalovirus viral loads in the 1 diagnosis of cytomegalovirus pneumonitis in infants Journal of Medical Virology 10.1002/jmv.24730

Hepatic tuberculosis in human immunodeficiency virus co-infected adults: a case series of South African adults, Lilishia Gounder1,2* , Pravikrishnen Moodley2 , Paul K. Drain3,4, Andrew J. Hickey5 and Mahomed-Yunus S. Moosa1,6, Gounder et al. BMC Infectious Diseases (2017) 17:115, DOI 10.1186/s12879-017-2222-2

Parboosing, R.; Chonco, L.; de la Mata, F. J.; Govender, T.; Maguire, G. E.; Kruger, H. G., Potential inhibition of HIV-1 encapsidation by oligoribonucleotide-dendrimer nanoparticle complexes. International Journal of Nanomedicine 2017:12 317–325

Salim Allana, Elena Shashkina, Barun Mathema, Nino Bablishvili, Nestani Tukvadze, N. Sarita Shah, Russell R. Kempker, Henry M. Blumberg, Pravi Moodley, Koleka Mlisana, James C.M. Brust, And Neel R. Gandhi, pnca Gene Mutations Associated with Pyrazinamide Resistance in Drug-Resistant Tuberculosis, South Africa And Georgia, Volume 23, Number 3—March 2017, Emerging Infectious Diseases • Www.Cdc.Gov/Eid • Vol. 23, No. 3, March 2017

N. Sarita Shah, M.D., M.P.H., Sara C. Auld, M.D., James C.M. Brust, M.D., Barun Mathema, Ph.D., Nazir Ismail, Ph.D., Pravi Moodley, M.D., Koleka Mlisana, M.D., Ph.D., Salim Allana, M.B., B.S., M.D., Angela Campbell, M.A., Thuli Mthiyane, M.Sc., Natashia Morris, M.Sc., Primrose Mpangase, B.A., Hermina Van Der Meulen, Shaheed V. Omar, Ph.D., Tyler S. Brown, M.D., Apurva Narechania, M.A., Elena Shaskina, Ph.D., Thandi Kapwata, M.Sc., Barry Kreiswirth, Ph.D., And Neel R. Gandhi, M.D. Transmission Of Extensively Drug-Resistant Tuberculosis In South Africa, N Engl J Med 2017; 376:243-253 19 January 2017, doi: 10.1056/Nejmoa1604544

Patent:

South African Provisional Patent Application No. 2017/00545 – “Novel DNA aptamers against CCL21 protein” filed 25 January 2017, R Parboosing and L Chonco